Medium Formulated at Normal Blood Glucose Level Without Serum and With CultureBoost-R™ (4N3-500-R) cell culture HIV Serologic Test (donor level

HIV Serologic Test (donor level HIV AB EIA) Negative HIV PCR Test (frozen cell pool by CLIA Licensed Clinical Lab) Negative Test of frozen cells for Mycoplasma spp

plated on AttachmentFactorTM-coated flask

the widely-used immortalized cell line RF/6A does not share hallmark endothelial cell characteristics

RocketFuel™ contains Cell Systems bovine growth factor and has been designed to supplement growth in the serum-free media environment

understanding and binding acceptance of these restrictions on behalf of the recipient Institution/Agency/Organization

Medium Formulated at Normal Blood Glucose Level Without Serum and With CultureBoost-R™ (4N3-500-R) cell culture HIV Serologic Test (donor levelMedium Kit Formulated at Normal Blood Glucose Level Without Serum and With CultureBoost R Kit consists of 500mL Cell Systems Normal Glucose Medium [No Serum] (glucose concentration = 0. 9008g L or 5mM), 10mL Attachment Factor (extracellular matrix reagent for coating flasks) and 10mL CultureBoost R growth supplement. This medium has been formulated at normal human plasma glucose level and becomes a complete medium with the addition of 5 20% serum (not